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. Author manuscript; available in PMC: 2014 Dec 6.
Published in final edited form as: J Proteome Res. 2013 Oct 3;12(12):5395–5409. doi: 10.1021/pr400818c

Figure 5.

Figure 5

Endogenous Tbx20 interacts with TLE1/3 in mouse embryonic hearts. (A) RT-PCR analysis of TLE family members and cardiac-specific markers Nkx2.5 and Tbx20 in E10.5 heart tissue. All samples derived from embryonic hearts dissected at E10.5. (B,C) Section in situ hybridization analysis for Tbx20 expression on a cryosection of an E10.5 heart. Neighboring sections from the same embryonic heart were used to assess TLE1 (D,E) and TLE3 (F,G) expression. (H) 25 hearts were dissected, and endogenous Tbx20 complexes were isolated with an antibody against Tbx20, analyzed by SDS-PAGE, and immunoblotted with antibodies against TLE1 and TLE3. In parallel and as a control, a mock immunoprecipitation was performed in the absence of Tbx20 antibody. ra, right atrium; la, left atrium; rv, right ventricle; lv, left ventricle.