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A subset of PT-derived cells internalize apical albumin with high efficiency. Filter-grown PTCs were incubated with 40 μg/ml Alexa Fluor 647-albumin (pseudocolored green) added apically for 30 min, fixed, and then processed to detect γ-GT (red). An individual optical section for γ-GT, a maximum projection of albumin distribution, and merged images are shown. Note that cells with robust albumin internalization express comparatively low levels of γ-GT. Scale bar, 10 μm.
