Fig. 5.

Rab11a-positive compartments in PTCs are highly dynamic. PTCs cultured on MatTek dishes were transfected with cDNA encoding SNAP-tagged Rab11a. After 2 days, cells were incubated with Cell-SNAP TMR-Star to label the SNAP tag, washed, and then imaged by live-cell microscopy to visualize Rab11a dynamics. Rab11a localizes to multiple variously sized spherical compartments that are highly dynamic. Left: a representative cell. Scale bar, 7.5 μm. Right: time-lapse (0–24 s) images of a tubule emanating from the Rab11a-positive structure enclosed in the box in the image at left. Scale bar, 1 μm. (Movie from which these images are derived is available in Supplemental Material.)