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. 2013 Dec 4;306(5):C450–C459. doi: 10.1152/ajpcell.00348.2013

Fig. 7.

Fig. 7.

Functional characteristics of Fpn-mediated efflux of 57Co and 65Zn. A: 57Co efflux rate constants (k) in control oocytes (gray) and oocytes expressing Fpn-EGFP (black) as a function of injectate 57Co concentration (n = 11–16 per group). Two-way ANOVA revealed an interaction (P < 0.001); Fpn differed from control at every concentration (P < 0.001). B: 57Co efflux rate constants (k) as a function of temperature in control oocytes (gray) and oocytes expressing Fpn-EGFP (black) (n = 10–12 per group) (injectate [57Co] = 0.5 μM). Data were fit by Eq. 3 for Fpn: Ea = 20.7 ± 1.4 kcal/mol, lnA = 37.0 ± 2.3 (r2 = 0.77, P < 0.001, n = 68); and control: Ea = 27.9 ± 6.3 kcal/mol, lnA = 45.7 ± 10.7 (r2 =0.24, P < 0.001, n = 62). Ea did not differ between Fpn and control (P = 0.25). C: 65Zn efflux rate constants (k) in control oocytes (gray) and oocytes expressing Fpn-EGFP (black) as a function of injectate 65Zn concentration (n = 10–18 per group). Two-way ANOVA revealed an interaction (P < 0.001); Fpn differed from control at every concentration (P < 0.001) except 0.5 μM (P = 1.0). D: 65Zn efflux rate constants (k) as a function of temperature in control oocytes (gray) and oocytes expressing Fpn-EGFP (black) (n = 9–12 per group) (injectate [65Zn] = 50 μM). Data were fit by Eq. 3 for Fpn: Ea = 20.5 ± 1.8 kcal/mol, lnA = 35.7 ± 3.1 (r2 = 0.67, P < 0.001, n = 65); and control: Ea = 6.9 ± 2.9 kcal/mol, lnA = 10.6 ± 4.9 (r2 =0.080, P < 0.021, n = 66). Ea differed between Fpn and control (P < 0.001).