Fig. 3.
Glioma cells display a pHe-sensitive whole cell conductance. A: voltage-clamp recordings from D54 human glioma cells in pHe 6.0, 7.4, and 8.8 bath solutions. B: pHe-sensitive currents after barrel application of pHe 6.0 or 8.8 from a control of pHe 7.4. C: representative traces of whole cell currents from D54 glioma cells in pHe 6.0, 7.4, and 8.8 bath solutions. D: whole cell current-clamp recordings from representative D54 and U251 human glioma cells, along with cultured cortical astrocytes (6.0A and 8.8A). E: quantification of pHe-sensitive current-clamp depolarizations and hyperpolarizations in D54 glioma cells and cortical astrocytes. F: representative current-clamp trace of D54 glioma cell with reversible acid-sensing ion channel blockade using 10 μM benzamil. *P < 0.05; **P < 0.01; ***P < 0.001.