Fig. 4.

Cx50^−/− fibers show a much larger increase in inward holding current in response to removal of external calcium than mouse lenses lacking both Cx50 and Cx46 (dKO) fibers. A: representative currents, obtained from a Cx50^−/− fiber in response to changing external divalent cation concentrations to the indicated values. The fiber was continuously held at a holding potential of −60 mV throughout the experiment. The dashed line indicates zero current. B: bar graph summarizing the holding current data at −60 mV in the presence of different concentrations of Mg²⁺ and Ca²⁺ for the experiment shown in A. C: representative current response obtained from a dKO fiber at −60 mV in response to changing external divalent cations concentrations to the indicated values. D: bar graph summarizing the holding current data at −60 mV in the presence of different concentrations of Mg²⁺ and Ca²⁺ for the experiment shown in C.