Fig. 5.

dKO fibers show reduced inward holding current and basal current fluctuations at −60 mV. A: population response of holding current between Cx50^−/− (black bars) and dKO fibers (gray bars). Data are means ± SE. Results are based on data obtained from 6 Cx50^−/− fibers and 8 dKO fibers. *P < 0.001, compared with dKO by Student's t-test. **P < 0.01, compared with dKO by Student's t-test. B: sample current traces for representative fiber cells isolated from Cx50^−/− and dKO lenses at −60 mV in the presence of 0 extracellular Ca²⁺ concentration and 2 mM extracellular Mg²⁺ concentration. C: population comparison of the basal current fluctuations at −60 mV showed an increase in mean current fluctuations in Cx50^−/− fibers relative to dKO fibers in the presence of divalent cations. The size of the current fluctuations about the mean was quantified by calculating the standard deviation of a 1.5-s segment of the current trace. *P < 0.001, compared with dKO by unpaired Student's t-test.