Figure 9.

Effect of (+)-PTZ on phosphorylation of p38 in LPS-activated microglia. (A) Retinal microglia cells were incubated with 1 μg/mL LPS at 37°C for 1, 3, 6, and 24 hours in the presence or absence of (+)-PTZ (3 μM, 30-minute pretreatment followed by cotreatment). Lysates were prepared from the cells and analyzed by Western blot using the indicated antibodies. Phosphorylation of p38 was increased by treatment with LPS at the 1-, 3-, and 6-hour time points. The LPS-induced increase in phosphorylation of p38 was not reliably changed by (+)-PTZ pretreatment. (B) Results from three experiments were quantified by densitometry (ImageJ).