Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 2;211(6):1063–1078. doi: 10.1084/jem.20132063

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Liu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — BLT2 is expressed in epidermal keratinocytes, and the ligand 12-HHT is produced in coagulated blood and wound exudate, in a COX/TxA₂S-dependent manner. (A and B) Relative BLT2 mRNA levels were measured by Q-PCR in mouse primary epidermal keratinocytes and dermal fibroblasts (A; n = 3 mice per group) and NHEKs and normal human dermal fibroblasts (NHDFs; B; n = 3 experimental replicates, P = 0.16, unpaired Student’s t test). (C) Immunohistochemical staining with anti–mouse BLT2 antibody and control rabbit IgG is shown in normal (uninjured) mouse skin. Arrows, BLT2 signals; arrowheads, nonspecific staining. Bars, 100 µm. (D) Immunohistochemical staining with (right) or without (left) anti–human BLT2 antibody is shown in normal (uninjured) human skin. Bars, 100 µm. (E) Biosynthesis and proposed mechanism of action of 12-HHT. (F and G) 12-HHT was quantified in mouse serum (F) and mouse wound fluid (G) by LC-MS/MS (n = 3–5 mice per group). Data represent the mean ± SEM. **, P < 0.01; *, P < 0.05; N.S., not significant (A and B, unpaired Student’s t test; F, one-way ANOVA with Bonferroni post hoc tests; G, two-way ANOVA with Bonferroni post hoc tests). All the results are representative of at least two independent experiments.