Figure 5.

12-HHT and a synthetic BLT2 agonist enhance primary keratinocyte migration. (A) BrdU incorporation was assessed in primary epidermal keratinocytes obtained from WT and BLT2 KO mice (n = 5 mice per group). (B–D) Keratinocytes were cultured to confluency, mechanically wounded by scratching, and then incubated in medium containing the indicated reagents. (B) Representative fields show the wound gap filled by WT and BLT2 KO primary keratinocytes cultured in the presence of 1 nM 12-HHT at 0 and 60 h after scratching. Lines indicate remaining gap. (C and D) Quantification of mouse primary keratinocyte (C, n = 9 experimental replicates) and NHEK (D, n = 12 experimental replicates) migration. Data represent the mean ± SEM. **, P < 0.01; N.S., not significant (A, unpaired Student’s t test; C and D, two-way ANOVA). All the results are representative of at least two independent experiments.