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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: Sci Transl Med. 2011 Nov 2;3(107):107ra109. doi: 10.1126/scitranslmed.3003062

Fig. 5.

Fig. 5

The priming effect of nicotine on cocaine in CBP+/− mice and mice treated with theophylline. (A1–A2) LTP measurement in CBP+/− mice and wild-type littermate controls treated with 7 days of nicotine; acute cocaine; and 7 days of nicotine followed by cocaine injection (n = 5–8). (A3) Histogram summary of changes in LTP amplitude at 180 min after HFS in different groups of mice (as shown in A1–A2). (A4) Input-output curve comparing CBP+/− mice and their wild-type littermates. (A5) Histone H4 (K5–16) tail acetylation in striatal lysates of CBP+/− mice and wild-type littermates after 7 days of nicotine exposure (n=4 per group). (B1) LTP measurement in mice treated with theophylline for 7 days; mice treated with theophylline followed by acute cocaine; mice treated with cocaine alone; and controls (n = 6–10 in each group). (B2) Histogram summary of changes in LTP amplitude at 180 min after HFS in different groups of mice (as shown in B1). (B3 and B4) Immunoblots of striatal lysates from mice treated for 7 days with theophylline (200 mg/liter in drinking water) probed with antibodies against acetylated histone H3 (K9) and acetylated histone H4 (K5 to K16) (B3), and antibodies against specific acetylated lysine residues on histone H4 (K5, K8, K12, and K16) (B4) (n = 4 per group). (B5) Real-time PCR measuring FosB expression in animals treated with local theophylline (0.2 mM) infused into the NAc for 7 days compared with controls (n = 5 per group). Groups as shown in B1. *P < 0.05.