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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: Anal Chem. 2011 Nov 7;83(23):9138–9145. doi: 10.1021/ac202335u

Figure 1.

Figure 1

Assay workflow. (A) Particles bearing aptamer probe are synthesized using SFL. The code stream contains 0.6% rhodamine acrylate. The schematic shows a particle bearing the graphical code “02223”. Particles with additional chemistries can be synthesized by altering the number of streams in the channel. (B) Particles are used to detect thrombin using a sandwich assay in which the reporting ligand is biotinylated to faciliate labeling with SA-PE. The two reporting ligands shown are a DNA aptamer (HTQ-3′) and a polyclonal antibody against thrombin (PAb-T). (C) Fluorescently labeled complexes are analyzed using either flow-through scanning or static imaging. Custom MATLAB scripts are used to extract individual particle profiles from scan data and images. Static particle image shown is false-colored.