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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: Anal Chem. 2011 Nov 7;83(23):9138–9145. doi: 10.1021/ac202335u

Figure 4.

Figure 4

Multiplexing demonstration. 100 pM thrombin and 0.667 pM IL-2 were detected simultaneously (M) using different particles. Assays were performed using both thrombin detection ligands (1:PAb-T, 2: HTQ-3′) and an IL-2 detection antibody (PAb-IL2). Reporter steps were carried out in optimal buffers (PBST for PAb-T and 10 mM phosphate with 10 mM KCl and 0.1% T-20 for HTQ-3′). Signals are compared side by side with target signal attained using a singleplex detection for both targets (S). Target signal is unaltered in multiplexed format and the HTQ-3′ buffer does not have an inhibitory effect on IL-2 reporter binding.