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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: Anal Chem. 2011 Nov 7;83(23):9138–9145. doi: 10.1021/ac202335u

Figure 6.

Figure 6

Evaluation of the effect of spacers with the HTDQ probe. Gradient particles were synthesized using HTDQ, HTDQ with a 6-thymine spacer at the 5′ end (HTDQ-6T), and HTDQ with a 12-thymine spacer at the 5′ end (HTDQ-12T). (A) Static image of a gradient particle incubated with 100 pM thrombin and 10 nM PAb-T. Longer thymine spacers lead to higher signal. (B) Bar graph showing the fold increase in signal when HTDQ-12T (orange) and HTDQ-6T (green) regions are compared to the HTDQ region, over a range of thrombin concentrations (10 pM, 50 pM, 100 pM, 500 pM). All particles were incubated with 10 nM PAb-T.