TABLE 1.

Sensitivities of ORF1 LAMP and PCR assays

N. gonorrhoeae genome copy no.	LAMP time to amplification (min)a	PCR result
2 × 105	17	+
2 × 104	18.6	+
2 × 103	20.7	+
2 × 102	25	+
20	27.3	+
2	No amplification	−
0	No amplification	−

^aAverage times to amplification of ORF1 LAMP reactions containing 2 × 10⁵, 2 × 10⁴, 2 × 10³, 2 × 10², 20, or 2 copies of the N. gonorrhoeae genome. Time to amplification was taken as the time point that the fluorescent signal exceeded a manually set threshold line on the Tube Scanner Studio software package (Qiagen). A LAMP reaction containing 5 μl of water instead of DNA was used as a negative control. ORF1 PCRs were carried out from the same DNA dilutions, and the products were separated using electrophoresis and then were visualized.