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. 2014 Jun;52(6):2163–2165. doi: 10.1128/JCM.00314-14

TABLE 1.

Sensitivities of ORF1 LAMP and PCR assays

N. gonorrhoeae genome copy no. LAMP time to amplification (min)a PCR result
2 × 105 17 +
2 × 104 18.6 +
2 × 103 20.7 +
2 × 102 25 +
20 27.3 +
2 No amplification
0 No amplification
a

Average times to amplification of ORF1 LAMP reactions containing 2 × 105, 2 × 104, 2 × 103, 2 × 102, 20, or 2 copies of the N. gonorrhoeae genome. Time to amplification was taken as the time point that the fluorescent signal exceeded a manually set threshold line on the Tube Scanner Studio software package (Qiagen). A LAMP reaction containing 5 μl of water instead of DNA was used as a negative control. ORF1 PCRs were carried out from the same DNA dilutions, and the products were separated using electrophoresis and then were visualized.