TABLE 1.
Data summary of MS-H-based flagellar typing of monophasic Salmonella reference strains
| S. enterica serovar | Antigens | No. of strains | Top hita (top hit/no. of tests)b |
|---|---|---|---|
| Enteritidis | 9,12:g,m:− | 3 | g,m (6/6) |
| 4,5,12:i:− | 4,5,12:i:− | 1 | i (2/2) |
| Derby | 4,12:f,g:− | 1 | f,g (2/2) |
| Monophasic Paratyphi B var. Java | 4,12:b:− | 2 | b (4/4) |
| Paratyphi A | 2,12:a:− | 2 | a (4/4) |
| Monophasic 4,[5],12,[27]:−:1,2 | 4,5,12:−:1,2 | 1 | 1,2 (2/2) |
| Alachua | 35:z4,z23:− | 1 | z4,z23 (2/2) |
| Tennessee | 6,7:z29:− | 1 | z29 (2/2) |
| Oranienburg | 6,7,14:m,t:− | 4 | m,t (6/8); m,p,t,u (2/8) |
| Typhi | 9,12:d:− | 1 | d (2/2) |
a
The top hit is the identified protein showing the highest Mascot score (signifying identification confidence) and emPAI value (signifying protein coverage, equal to 10 to the power of [the number of observed peptides divided by the number of observable peptides] minus one [reference 26]) and is regarded as the correct flagellar type.
b
Top hit/no. of tests is how often the top hit was identified as such during MS-H testing.