TABLE 2.
Data summary of MS-H-based flagellar typing of multiphasic Salmonella reference strains
| S. enterica serovar | Antigens | No. of strains | H antigen top hit (top hit/no. of tests)a |
Note | |
|---|---|---|---|---|---|
| Phase 1 | Phase 2 | ||||
| Typhimurium | 4,5,12:i:1,2 | 2 | r,i (2/4) | 1,2 (2/4) | |
| b (2/4) | 1,5 (2/4) | ||||
| Heidelberg | 4,12:r:1,2 | 3 | r (3/6) | 1,2 (2/6) | |
| r,i (2/6) | 1,5 (4/6) | ||||
| i (1/6) | |||||
| Typhi | 9,12:d:z66 | 1 | d (2/2) | z66 (2/2) | |
| Infantis | 6,7:r:1,5:z49 | 1 | r (2/2) | 1,5 (2/2) | z49 sequence unavailable |
| Infantis | 6,7:r:1,5 | 2 | r (3/4) | 1,5 (2/4) | |
| r,i (1/4) | 1,2 (1/4) | ||||
| 1,(5),7 (1/4) | |||||
| Saintpaul | 4,12:e,h:1,2 | 3 | e,h (5/5) | 1,2 (3/5) | |
| 1,5 (1/5) | |||||
| 1,6 (1/5) | |||||
| Hadar | 6,8:z10:e,n,x | 3 | z10 (5/5) | e,n,x (4/5) | Once monophasic |
| Newport | 6,8:e,h:1,2 | 2 | e,h (4/4) | 1,2 (4/4) | |
| Thompson | 6,7:k:1,5 | 3 | k (6/6) | 1,5 (4/6) | Once monophasic |
| 1,2,7 (1/6) | |||||
| Javiana | 9,12:l,z28:1,5 | 1 | l,w (1/2) | ||
| l,v (1/2) | 1,5 (2/2) | ||||
| Uganda | 3,15:l,z13:1,5 | 1 | l, z13 (2/2) | enx15 (1/2) | Once monophasic |
| Schwarzengrund | 4,12,27:d:1,7 | 1 | d (2/2) | 1,5 (1/2) | |
| 1,7 (1/2) | |||||
| Panama | 9,12:l,v:1,5 | 1 | l,v (2/2) | 1,5 (2/2) | |
| Kentucky | 8,20:i:z6 | 2 | r,i (4/4) | z6 (4/4) | |
a
Top hit/no. of tests is how often the top hit was identified as such during MS-H testing. The top hit is the identified protein showing the highest Mascot score (signifying identification confidence) and emPAI value (signifying protein coverage, equal to 10 to the power of [the number of observed peptides divided by the number of observable peptides] minus 1 [reference 26]) and is regarded as the correct flagellar type.