TABLE 4.
Species (no. of isolates tested) and test method | % of MICs by categorya |
CA (%) | % VME | % ME | % Minor errors | ||
---|---|---|---|---|---|---|---|
S | SDD | R | |||||
C. albicans (215) | |||||||
Vitek 2 | 93.0 | 0.5 | 6.5 | ||||
BMDb | 94.4 | 3.3 | 2.3 | 94.9 | 0.0 | 2.3 | 2.8 |
C. glabrata (181) | |||||||
Vitek 2 | 71.8 | 28.2 | |||||
BMD | 85.6 | 14.4 | 87.8 | 0.0 | 0.0 | 12.2 | |
C. parapsilosis (109) | |||||||
Vitek 2 | 85.3 | 0.9 | 13.8 | ||||
BMD | 83.5 | 3.7 | 12.8 | 94.5 | 1.8 | 0.9 | 2.8 |
C. tropicalis (107) | |||||||
Vitek 2 | 86.0 | 1.9 | 12.1 | ||||
BMD | 87.9 | 6.5 | 5.6 | 90.6 | 0.0 | 4.7 | 4.7 |
C. krusei (30) | |||||||
Vitek 2 | 100 | ||||||
BMD | 100 | 100 | 0.0 | 0.0 | 0.0 | ||
C. lusitaniae (28) | |||||||
Vitek 2 | 82.1 | 17.9 | |||||
BMD | 96.4 | 3.6 | 85.7 | 0.0 | 14.3 | ||
C. dubliniensis (13) | |||||||
Vitek 2 | 84.6 | 15.4 | |||||
BMD | 100 | 0.0 | 84.6 | 0.0 | 15.4 | ||
C. guilliermondii (8) | |||||||
Vitek 2 | 100 | 0.0 | |||||
BMD | 100 | 0.0 | 100 | 0.0 | 0.0 | ||
C. pelliculosa (4) | |||||||
Vitek 2 | 100 | 0.0 | |||||
BMD | 100 | 0.0 | 100 | 0.0 | 0.0 | ||
C. kefyr (3) | |||||||
Vitek 2 | 66.7 | 33.3 | |||||
BMD | 100 | 0.0 | 66.7 | 0.0 | 33.3 | ||
C. neoformans (44) | |||||||
Vitek 2 | 84.1 | 15.9 | |||||
BMD | 90.9 | 9.1 | 84.1 | 4.5 | 11.4 |
The CBPs for S, SDD, and R, respectively, were those of the CLSI of fluconazole for C. albicans, C. tropicalis, and C. parapsilosis (S, ≤2 μg/ml; SDD, 4 μg/ml; R, ≥8 μg/ml) and C. glabrata (SDD, ≤32 μg/ml; R, ≥64 μg/ml). There are no CBPs of fluconazole for C. krusei; all isolates are categorized as R irrespective of the MIC. Because of the lack of CBPs, the ECV was used to determine fluconazole S (WT) and R (non-WT) of C. lusitaniae (≤1 and ≥2 μg/ml), C. dubliniensis (≤0.5 and ≥1 μg/ml), C. guilliermondii (≤8 and ≥16 μg/ml), C. pelliculosa (≤4 and ≥8 μg/ml), C. kefyr (≤1 and ≥2 μg/ml), and C. neoformans (≤16 and ≥32 μg/ml).
The CLSI BMD test result was read at either 24 h (Candida species) or 72 h (C. neoformans) of incubation.