FIG 1.

Agarose gels of PCR amplicons with AFCYP98F and AFCYP98R primers from 7 A. fumigatus isolates (lanes 1 to 7) (a) and RFLP patterns obtained from 8 triazole-resistant (lanes 1 to 8) and 6 triazole-susceptible (lanes 9 to 14) A. fumigatus isolates (b). The positions of migration of diagnostic fragments of 289 bp (for the L98H mutation) and 189 bp (for the wild-type codon at cyp51A98) are indicated by arrows. The minor band at 350 bp in some lanes represents the undigested full-length amplicon. In both panels, lane M is a 100-bp DNA ladder, and the positions of migration of 100-bp, 300-bp, and 600-bp fragments are marked.