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Agarose gel of PCR products obtained with AFCYPPF and AFCYPPR primers from 8 A. fumigatus isolates. The amplicon of ∼139 bp in lanes 1, 2, 5, and 6 indicates the presence of TR34, while the amplicon of ∼105 bp in lanes 3, 4, 7, and 8 indicates the absence of TR34 (wild-type sequence) in the promoter region of cyp51A. Lane M is a 100-bp DNA ladder, and the positions of migration of 100-bp, 300-bp, and 600-bp fragments are marked.
