TABLE 1.

Primers and probes for three PCR-based detection methods

Primer	Primer and probe sequences (5′ to 3′)a	Target species or genus	Amplicon size (bp)	Reference
Nested PCR-1
rPLU1_F	TCA AAG ATT AAG CCA TGC AAG TGA	Plasmodium	∼1,670	27
rPLU5_R	CCT GTT GTT GCC TTA AAC TTC
rPLU3_F	TTT TTA TAA GGA TAA CTA CGG AAA AGC TGT	Plasmodium	240	27
rPLU4_R	TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC
rFAL1_F	TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT	P. falciparum	206	27
rFAL2_R	ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC
rVIV1_F	CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC	P. vivax	121	27
rVIV1_R	ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA
rMAL1_F	ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC	P. malariae	145	27
rMAL2_R	AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA
rOVA3_F	CGG GGA AAT TTC TTA GAT TGC	P. ovale	456	27
rOVA4_R	GAG AAA CAG CAT GAA TTG CG
Nested PCR-2
P1_F	ACG ATC AGA TAC CGT CGT AAT CTT	Plasmodium	∼110	17
P2_R	GAA CCC AAA GAC TTT GAT TTC TCA T
F2_R	CAA TCT AAA AGT CAC CTC GAA AGA TG	P. falciparum	∼110	17
V1_R	CAA TCT AAG AAT AAA CTC CGA AGA GAA A	P. vivax	∼110	17
M1_R	GGA AGC TAT CTA AAA GAA ACA CTC ATA T	P. malariae	∼110	17
O2_R	ACT GAA GGA AGC AAT CTA AGA AAT TT	P. ovale	∼110	17
Real-time PCR
Spp_F	GTT AAG GGA GTG AAG ACG ATC AGA TA	Plasmodium	∼157	26
Spp_R	AAC CCA AAG ACT TTG ATT TCT CAT AAG
Pan-spp probe	FAM-TCG TAA TCT TAA CCA TAA AC
Pf-1_F	ATT GCT TTT GAG AGG TTT TGT TAC TTT	P. falciparum	95	26
Pf-2_R	GCT GTA GTA TTC AAA CAC AAT GAA CTC AA
Pf-probe	FAM-CAT AAC AGA CGG GTA GTC AT
Pv-1_F	CGCTTCTAGCTTAATCCACATAACTG	P. vivax	142	19
Pv-2_R	AATTTACTCAAAGTAACAAGGACTTCCAAG
Pv-probe	HEX-CGCATTTTGCTATTATGT
Pm-1_F	AGT TAA GGG AGT GAA GAC GAT CAG A	P. malariae	166	26
Pm-2_R	CAA CCC AAA GAC TTT GAT TTC TCA TAA
Pm-probe	FAM-ATG AGT GTT TCT TTT AGA TAG C
Po-1_F	CCG ACT AGG TTT TGG ATG AAA GAT TTT T	P. ovale	∼114	26
Po-2_R	CAA CCC AAA GAC TTT GAT TTC TCA TAA
Po-probe	HEX-CGA AAG GAA TTT TCT TAT T
GAPDH_F	CCT CCC GCT TCG CTC TCT	Homo sapiens	65	26
GAPDH_R	GCT GGC GAC GCA AAA GA
GAPDH-probe	FAM-CCT CCT GTT CGA CAG TCA GCC GC

^aFAM and HEX denote fluorescent dyes.