TABLE 1.
Primer | Primer and probe sequences (5′ to 3′)a | Target species or genus | Amplicon size (bp) | Reference |
---|---|---|---|---|
Nested PCR-1 | ||||
rPLU1_F | TCA AAG ATT AAG CCA TGC AAG TGA | Plasmodium | ∼1,670 | 27 |
rPLU5_R | CCT GTT GTT GCC TTA AAC TTC | |||
rPLU3_F | TTT TTA TAA GGA TAA CTA CGG AAA AGC TGT | Plasmodium | 240 | 27 |
rPLU4_R | TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC | |||
rFAL1_F | TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT | P. falciparum | 206 | 27 |
rFAL2_R | ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC | |||
rVIV1_F | CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC | P. vivax | 121 | 27 |
rVIV1_R | ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA | |||
rMAL1_F | ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC | P. malariae | 145 | 27 |
rMAL2_R | AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA | |||
rOVA3_F | CGG GGA AAT TTC TTA GAT TGC | P. ovale | 456 | 27 |
rOVA4_R | GAG AAA CAG CAT GAA TTG CG | |||
Nested PCR-2 | ||||
P1_F | ACG ATC AGA TAC CGT CGT AAT CTT | Plasmodium | ∼110 | 17 |
P2_R | GAA CCC AAA GAC TTT GAT TTC TCA T | |||
F2_R | CAA TCT AAA AGT CAC CTC GAA AGA TG | P. falciparum | ∼110 | 17 |
V1_R | CAA TCT AAG AAT AAA CTC CGA AGA GAA A | P. vivax | ∼110 | 17 |
M1_R | GGA AGC TAT CTA AAA GAA ACA CTC ATA T | P. malariae | ∼110 | 17 |
O2_R | ACT GAA GGA AGC AAT CTA AGA AAT TT | P. ovale | ∼110 | 17 |
Real-time PCR | ||||
Spp_F | GTT AAG GGA GTG AAG ACG ATC AGA TA | Plasmodium | ∼157 | 26 |
Spp_R | AAC CCA AAG ACT TTG ATT TCT CAT AAG | |||
Pan-spp probe | FAM-TCG TAA TCT TAA CCA TAA AC | |||
Pf-1_F | ATT GCT TTT GAG AGG TTT TGT TAC TTT | P. falciparum | 95 | 26 |
Pf-2_R | GCT GTA GTA TTC AAA CAC AAT GAA CTC AA | |||
Pf-probe | FAM-CAT AAC AGA CGG GTA GTC AT | |||
Pv-1_F | CGCTTCTAGCTTAATCCACATAACTG | P. vivax | 142 | 19 |
Pv-2_R | AATTTACTCAAAGTAACAAGGACTTCCAAG | |||
Pv-probe | HEX-CGCATTTTGCTATTATGT | |||
Pm-1_F | AGT TAA GGG AGT GAA GAC GAT CAG A | P. malariae | 166 | 26 |
Pm-2_R | CAA CCC AAA GAC TTT GAT TTC TCA TAA | |||
Pm-probe | FAM-ATG AGT GTT TCT TTT AGA TAG C | |||
Po-1_F | CCG ACT AGG TTT TGG ATG AAA GAT TTT T | P. ovale | ∼114 | 26 |
Po-2_R | CAA CCC AAA GAC TTT GAT TTC TCA TAA | |||
Po-probe | HEX-CGA AAG GAA TTT TCT TAT T | |||
GAPDH_F | CCT CCC GCT TCG CTC TCT | Homo sapiens | 65 | 26 |
GAPDH_R | GCT GGC GAC GCA AAA GA | |||
GAPDH-probe | FAM-CCT CCT GTT CGA CAG TCA GCC GC |
FAM and HEX denote fluorescent dyes.