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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: Nat Struct Mol Biol. 2009 Sep 20;16(10):1101–1108. doi: 10.1038/nsmb.1668

Figure 2.

Figure 2

N-terminomics reveals protease specific cleavage-sites. N-terminomic analysis of E. coli lysate treated with (a) human caspase-3 and (b) GluC reveals specific substrates and corresponding cleavage-sites. A tripartite criterion was used to identify genuine cleavage-sites of exogenous proteases from background proteolytic events inherent in E. coli lysate: Cleavage-sites must (1) not already be annotated as an endogenous site of proteolysis, (2) be only found in protease treated samples, and never in control samples, and (3) maintain the P1 aspartate/glutamate characteristic of each protease. See Table 1 for definitions.