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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: J Immunol. 2011 Aug 1;187(5):2181–2192. doi: 10.4049/jimmunol.1003460

Figure 4. MSP stimulation of primary macrophages induced Fos binding to the AP-1 site in the Arg1 promoter.

Figure 4

Primary peritoneal macrophages were stimulated with 100 ng/mL MSP or 10 ng/mL IL-4 and chromatin immunoprecipitation (ChIP) and subsequent qPCR was performed for A) Fos and B) Stat6. Data are presented as mean ± S.D., normalized to unstimulated macrophages, and are representative of three independent experiments. C) Day 4 thioglycollate-elicited macrophages and resident peritoneal macrophages from wild-type or Ron−/− mice were analyzed by flow cytometry for Ron expression. D) Thioglycollate-elicited macrophages were stimulated with 100 ng/ml MSP for the indicated times and lysates were immunoblotted for phosphorylated Erk. E) Thioglycollate-elicited macrophages were stimulated with 100 ng/mL MSP for 2 hours and assessed for Arg1 expression by qRT-PCR. F) Thioglycollate-elicited macrophages were stimulated with 100 ng/mL MSP for 2 hours and Fos binding to the AP1 site in the Arg1 promoter was assessed by ChIP and subsequent qPCR. Data are presented as mean ± S.D., normalized to unstimulated macrophages, and representative of two independent experiments.