Figure 2.

tbx5 morphants exhibit cardiac jogging and lefty2 expression defects. (a–a″) tbx5a morphant phenotypes. (b–b″) tbx5b morphants. (c–e″) Left, right and middle jog phenotypes obtained by co-injection of tbx5a and tbx5b MOs at 0.5 ng (c–c″), 1.5 ng (d–d″) or 3 ng (e–e″) of each MO. (f) Control (ctrl) morphant. (g) Quantification of the phenotypes. (h–j) lefty2 expression in control (h) and double-morphant (i,j) 22-somite stage embryos. (k) Quantification of the phenotypes. A χ² test has been used to assess significant differences between groups (**p < 0.001). All images are dorsal views with anterior to the top, and myl7 expression is used to highlight the developing heart tube in a–f.