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. 2014 Apr 30;4(4):140043. doi: 10.1098/rsob.140043

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — Eic1 and Eic2 depend on distinct Cnp1^CENP-A assembly factors for their association with centromeres. (a,b) Eic1 association with centromeres is dependent on Mis18, Mis16^{RbAp46/48/Hat2} and Scm3^HJURP, but is largely independent of Cnp1^CENP-A, Mis6^CENP-I/Ctf3, Mis12 and Eic2. qChIP analyses of Eic1-GFP association with centromeres in the indicated strains when grown at permissive (25°C) versus restrictive temperature (36°C) for 8 h (a), or when grown at 32°C (b). (c,d) Eic2 association with centromeres is dependent on Mis18, Mis16^{RbAp46/48/Hat2}, Scm3^HJURP, Cnp1^CENP-A, Mis6^CENP-I/Ctf3, Mis12 and Eic1. qChIP analyses of Eic2-GFP association with centromeres in the indicated strains when grown at permissive (25°C) versus restrictive temperature (36°C) for 8 h. Enrichment of cc2 DNA relative to the act1 locus is presented in (a,c,d). Enrichment of cc2 or cc1/3 DNA relative to the act1 locus is presented in (b). Error bars represent standard deviation between at least three biological replicates.