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. 2014 Apr 30;4(4):140043. doi: 10.1098/rsob.140043

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — Eic1 interacts with three essential subunits of the CCAN/Mis6/Ctf19 complex. (a) LC-MS/MS analysis of GFP-tagged Eic1 or Eic2 immunoprecipitates from S. pombe whole cell extracts identifies Fta7^CENP-Q/Okp1, Cnl2^Nkp2 and Mal2^CENP-O/Mcm21 as Eic1-interacting proteins. Average number of unique peptides reproducibly identified from three independent experiments is shown. (b) Eic1-GFP co-immunoprecipitates with Fta7-Myc and Cnl2-Myc. In the top panel, the asterisk (*) denotes the IgG heavy chain, and the hash tag (#) denotes a non-specific band.