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. 2004 Apr 1;23(9):1934–1942. doi: 10.1038/sj.emboj.7600198

Figure 3.

Figure 3

Importin α3- and α5-dependent nuclear import of p27. (A) Solution-binding assay. Flag-p27 was incubated with GST or GST-importin α's with (lanes 1–5) or without (lanes 6–10) HA-importin β in the presence of anti-Flag agarose. The same experiment was carried out using Flag-p27 (K165A/R166A) without importin β (lanes 11–15). The bound proteins were eluted and analyzed by SDS–PAGE. GST-importin α1, α3 and α5 are indicated as GST-α1, -α3 and -α5. HC: heavy chain and LC: light chain. (B) In vitro transport assay. Flag-p27 was applied to the in vitro reconstituted transport assay system supplemented with Ehrlich cytosol, or importin α/β with Ran and NTF2.