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. 2004 Apr 1;23(9):1934–1942. doi: 10.1038/sj.emboj.7600198

Figure 4.

Figure 4

Phosphorylation by Akt increased cytoplasmic p27. (A) HeLa cells were transfected with pcDNA-Flag-p27 (wild or T157A) together with empty vector (−) or pcDNA-Akt (CA or DN) at 1:9 ratio for 24 h. DNA was stained by Hoechst dye. CA, constitutively active form. DN, dominant-negative form. (B) Phosphorylation of p27 by Akt reduced importin α5 binding. 293T cells were transfected with pcDNA-HA-p27 (wild) and pcDNA-Flag-importin α5 (Flag-α5) together with empty vector (−) or pcDNA-Akt (CA). HA-p27 was immunoprecipitated (IP) and co-precipitated proteins were analyzed by Western blotting using anti-Flag or anti-HA antibody.