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. 2004 Apr 1;23(9):1943–1948. doi: 10.1038/sj.emboj.7600199

Figure 4.

Figure 4

Interdependent recruitment of Spt3 and Mot1 on the GAL1 promoter and the NPS. (A) Occupancy of the GAL1 and PGK1 promoters by Mot1. Chromatin immunoprecipitation was conducted using the indicated strains expressing Myc-tagged Mot1 and growing under repressive (GLU) or inducing (GAL) conditions. (B) Occupancy of the NPS and PGK1 promoter by Mot1. ChIP was performed in gcn4 (WT), gcn4gcn5 (gcn5) and gcn4spt3 (spt3) strains expressing the chimeric activator and Myc-tagged Mot1 and growing under repressive (MIN) or inducing (AT) conditions. (C) Occupancy of GAL1 and ADH1 promoters by Spt3. ChIP was performed in the indicated strains grown in galactose and containing the Myc-tagged version of Spt3. (D) Occupancy of the NPS and ADH1 promoter by Spt3. ChIP was performed in gcn4 (WT) and gcn4mot1-1 (mot1-1) strains expressing the chimeric activator and the Myc-tagged Spt3 and grown in histidine-depleted media. In all experiments numbers in the x-axis are as described in the legend of Figure 2C.