Table 2.
Active site mutants of SnoaL
| Enzyme variant | Specific activity (nmol/mg s) |
|---|---|
| Wild type | 21.1 |
| Phe15Tyr | 1.1 |
| Gln105Ala | 3.3 |
| Gln105Glu | Inclusion bodies |
| Asp121Ala | No detectable activity |
| Asp121Asn | Inclusion bodies |
Active site mutants of SnoaL
| Enzyme variant | Specific activity (nmol/mg s) |
|---|---|
| Wild type | 21.1 |
| Phe15Tyr | 1.1 |
| Gln105Ala | 3.3 |
| Gln105Glu | Inclusion bodies |
| Asp121Ala | No detectable activity |
| Asp121Asn | Inclusion bodies |