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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Exp Cell Res. 2014 Feb 8;324(1):40–53. doi: 10.1016/j.yexcr.2014.01.024

Figure 5. Effect of EGCG and NaB on the mRNA expression of specific epigenetic, cell cycle and cell proliferation modulators in RKO colon cancer cells.

Figure 5

Figure 5

RKO colorectal cancer cells were treated to 10 μM EGCG and 5 mM NaB and after a 48 h time period RNA was extracted. Real time assessment of mRNA expression of the various genes revealed that DNMT1 and survivin expression decreased significantly for the combination treatment as compared to EGCG and NaB alone (A, B). EGCG is a known DNMT1 inhibitor although its effects are more pronounced at the protein than mRNA level. However, HDAC1, p21, and NF-κB-p65 (A, B, C) increased several fold and the increase was significant when compared to the control and individual treatments. A significant increase in NF-κB-p65 was observed for both the NaB alone and the combination treatment. However, the combination treatment was lower as compared to the NaB alone treatment, and significant and relatively higher when compared to EGCG and the control. Data are in triplicates from three independent experiments. * < 0.05, ** < 0.01