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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Exp Cell Res. 2014 Feb 8;324(1):40–53. doi: 10.1016/j.yexcr.2014.01.024

Figure 7. Effect of the combinatorial compounds on the expression of p21, p53 and NF-κB-p65 from nuclear lysates; γ-H2AX, anti-acetylated H3, DNMT3A and DNMT3B from total protein lysates, in RKO colon cancer cells.

Figure 7

Figure 7

Nuclear proteins were analyzed in triplicates for p21, p53, and NF-κB-p65 in RKO colon cancer cells (Figure 7A) and total protein in triplicates was assessed for γ-H2AX and anti-acetylated H3 levels (Figure 7B). At 10 μM EGCG and 5 mM NaB combination, p21 levels increased in RKO colorectal cancer cells. An induction in p53 expression was also observed in the combination treatment. Thus, EGCG may enhance the effect of NaB by inducing p53 expression. NF-κB-p65 levels increased in the combination and NaB treatment when compared to the control and EGCG treatment. This increase may be associated with the double-strand breaks (DSBs) indicated by increased γ-H2AX levels (7B) and G2/M arrest as was found in the study. De novo methylation-associated epigenetic proteins DNMT3A and DNMT 3B levels also decreased for the combination and this was tested only in RKO colon cancer cells (Figure 7C). An increase in anti-acetylated H3 levels was also observed with the combination (Figure 7B), an epigenetic change probably associated by the decrease in HDAC1 levels and HDAC activity. * < 0.05, ** < 0.01.