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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Exp Cell Res. 2014 Feb 8;324(1):40–53. doi: 10.1016/j.yexcr.2014.01.024

Figure 8. p53-dependent induction of p21 by EGCG and NaB combination treatment in RKO colon cancer cells.

Figure 8

ChIP-PCR analysis of RKO cell p53-enrinched chromatin showed a significant upregulation of p21 in the combination treatment. Primers chosen were specific for the p21 promoter region. The increase in p53-enriched p21 levels in the combination is indicative of a p53-dependent mechanism of p21 induction in RKO colon cancer cells. The result is in agreement with the increase in mRNA and protein levels of p21 observed in the study. PCRs are from a single experiment repeated three times * < 0.05, ** < 0.01.