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Levels of meiotic DSBs in the set1Δ rad50S mutant (SK1 background). (A) DSBs at the DEP1-CYS3 locus were analyzed as in Figure 5. (B) FACS analysis were carried out to follow meiotic DNA replication in the same experiment as in (A) at the indicated times after shift in sporulation medium. (C) Quantification of the DSB fragments to parental fragment at the DEP1-CYS3 locus and the YCR047c locus. Closed diamonds, rad50S; open circles, set1Δ rad50S.
