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. 2014 Feb 26;35(6):1379–1388. doi: 10.1093/carcin/bgu050

Fig. 3.

Fig. 3.

Six1 overexpression in HKc/DR increases the expression of TGF-β receptors. (A) Levels of mRNA expression of TGF-β receptors TβRI, TβRII and TβRIII were determined by real-time PCR in HKc/DR-Ctrl (DR-Ctrl) and HKc/DR-Six1 (DR-Six1). Data were normalized to β-actin expression. Bars indicate standard deviation, and ***indicates statistically significant P values ≤ 0.001. (B) (Left panel) Western blot analysis of TβRI, TβRII and TβRIII in HKc/DR-Ctrl (DR-Ctrl) and HKc/DR-Six1 (DR-Six1). β-Actin was used as control for equal protein loading. Right panels: immunofluorescent staining of TβRI, TβRII and TβRIII (red) in HKc/DR-Ctrl (DR-Ctrl) and HKc/DR-Six1 (DR-Six1). Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). Images are shown at ×630 magnification.