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. 2014 Feb 7;35(6):1341–1351. doi: 10.1093/carcin/bgu042

Fig. 3.

Fig. 3.

FASN regulates the bioavailability of VEGF-A. (A) Secretion of VEGF121 and VEGF165 by CRC cell lines measured by ELISA. (B) Localization of VEGF-A (green) in HCT116 and HT29 cells with knockdown of FASN assessed by confocal microscopy. ×40 and ×60 (×4 zoom) magnification. (C) IHC staining of orthotopic HCT116 and HT29 colon tumor sections for VEGF-A. ×20 (insets ×180) magnification. (D) Localization of VEGF-A assessed in tumor sections by confocal microscopy. VEGF-A (red), smooth muscle actin (green), ×60 magnification. (E) The effect of FASN overexpression on secretion of VEGF121 and VEGF165 by SW480 cells measured by ELISA. (F) Expression of VEGF-A in SW480 cells with overexpression of FASN, ×40 magnification.