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Quantitation of gingerols: 6G, 8G, 10G and 6S were quantified employing LC-MS/MS with electrospray ionization in positive-ion mode. The presence of 6G (MRM: 277.2/177.2, RT: 5.8min), 8G (MRM: 305.2/137.2, RT: 9.4min), 10G (MRM: 333.2/137.2, RT: 14.6min) and 6S (MRM: 277.2/137.1, RT: 10.9min) were confirmed using analytical standards and quantified using calibration curve for each individual component (shown in the inset).
