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. 2014 Jan 8;35(6):1217–1227. doi: 10.1093/carcin/bgu006

Fig. 2.

Fig. 2.

Knockdown of the α6A subunit in human CRC cells. (A) Representative gel showing the results of a competitive reverse transcription–polymerase chain reaction for the detection of α6A and α6B transcripts in stably expressing shctl and shα6A Caco-2/15, DLD-1, T84 and HT29 cells. (B) qPCR using probes specific for total α6, α6A and α6B confirming the specificity of abolition of α6A variant expression by shα6A relative to shctrl; *P ≤ 0.05, **P ≤ 0.01, ANOVA, n = 3. (C) Representative WB for detection of α6A and α6B subunits in shctl- and shα6A-infected Caco-2/15, DLD-1, T84 and HT29 cells. Keratin 18 (K18) was used as loading control. Densiometric analysis of α6A and α6B protein levels in shctl- versus shα6A-infected cell populations; *P ≤ 0.05, **P ≤ 0.01, t-test, n = 3.