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Intracellular distribution of Ufm1 in HeLa cells. (A) HeLa cells were seeded on coverslips 24 h before fixation for immunostaining. Ufm1 was detected with anti-Ufm1 serum and visualized with Alexa 488 nm anti-rabbit antibody. (B) Immunocytochemical analysis was conducted as for (A), except that preimmune serum was used. Cells were observed using a fluorescence microscope. Magnification, × 400.
