FIG. 4.

Nissl staining 14 days post-traumatic brain injury (TBI). (A) Mosaic images of the vehicle and methylene blue (MB)-treated animals. The red box indicates the location of the zoom-in images. (B) Magnified images of a vehicle- and an MB-treated TBI animal obtained using a 60×objective. The red arrows indicate typical dark-stained neurons. (C) Bar graph of the number of dark-stained Nissl bodies for vehicle- and MB-treated animals (mean±SEM, n=6 each group, *p<0.01, ^##p<0.01). Color image is available online at www.liebertpub.com/neu