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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Mech Ageing Dev. 2013 Dec 18;135:33–40. doi: 10.1016/j.mad.2013.11.007

Figure 5. Analysis of the apoptotic potential of resting NK cells from young and aged mice.

Figure 5

(A) Freshly isolated bone marrow and spleen cells were stained intracellularly for caspace-3. Median Fluoresence Intensity (MFI) of expression for each NK cell subset, (n=5). (B) Freshly isolated bone marrow and spleen cells were stained intracellularly for Bcl-2. MFI of expression for each NK cell subset, (n=5). (C) Freshly isolated bone marrow and spleen cells were stained intracellularly for BIM. MFI of expression for each NK cell subset, (n=5). (D) Annexin staining for each NK subset for bone marrow and spleen cells incubated for 10 hrs without growth factors at 37°C, (n=10). Asterisks indicate statistical significant difference between the two age groups, t-test, p < 0.05.