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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: J Mol Biol. 2014 Jan 30;426(8):1766–1785. doi: 10.1016/j.jmb.2014.01.006

Figure 1. SRAP expression level and localization in MCF-7 cells.

Figure 1

(a) SRAP quantitative western blot. Left: Lanes 1-6, purified SRAP (13-236) protein; lane 7, lysate from ~120,000 MCF-7 cells. SRAP was probed with Abcam antibody ab72552. Right: plot of western signal versus amount of SRAP. Open circle, signal from cell lysate. (b) Immunofluorescence analysis of SRAP in MCF-7 cells. SRAP was probed with Abcam antibodies ab72407 (top panels) or ab72552 (bottom panels). The signal specificity was confirmed by knocking down SRAP with pool 2 SRA siRNA (middle rows) as well as by competition with recombinant SRAP (13-236) protein (bottom rows).