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. Author manuscript; available in PMC: 2014 Oct 12.
Published in final edited form as: Int J Cardiol. 2013 Jul 29;168(5):4643–4651. doi: 10.1016/j.ijcard.2013.07.190

FIGURE 1.

FIGURE 1

TAC1, but not NK-1 receptor expression corresponds with increased blood pressure in the SHR. A. Systolic blood pressure was measured every 4 weeks in WKY (circles with solid line, n=8), SHR (squares with dashed line, n=8), and SHR treated with L732138 (triangles with dotted line, n=8). Systolic blood pressure measurements are plotted against age of rat at time of measurement. Error bars represent SEM, ‡p < 0.005, 2-way ANOVA with a Bonferonni post-test for SHR and SHR+L732138 groups compared to the WKY. B. Relative fold expression of TAC1 mRNA was measured by qRT-PCR from left ventricular tissue in 8, 12, 16, 20, and 24-week old WKY (circles with solid line, n=5) and SHR (squares with dashed line, n=5). TAC1 Ct values were normalized to β-actin Ct values and fold expression were determined using the ΔΔCt method. Error bars represent SEM, †p < 0.01 and ‡p < 0.005 using a 2-way ANOVA between WKY and SHR with a Bonferonni post-test. C. Relative fold expression of Nk-1 receptor mRNA was measured by qRT-PCR and normalized to β-actin expression in left ventricular tissue isolated at 8, 12, 16, 20, and 24-weeks of age in WKY (black bars, n=5-8) and SHR (white bars, n=5-8). Nk-1 receptor mRNA was measured from the left ventricle of 24-week old SHR treated with L732138 (grey bar, n=5). Error bars represent SEM. **p < 0.01 and ***p < 0.005 using a 2-way ANOVA with a Bonferroni post-test. D. NK-1 receptor and GAPDH were detected by western blot from left ventricular tissue isolated at 8, 12, 16, and 20-week old WKY and SHR. E. Band densities from NK-1 receptor were normalized to band densities of GAPDH. The black bars represent WKY samples (n=4) and the white bars represent SHR samples (n=4). **p < 0.01 and ***p < 0.005 using a 2-way ANOVA with a Bonferonni post-test. F. NK-1 receptor and GAPDH expression were detected by western blot from left ventricular tissue isolated at 24-weeks from WKY, SHR, and SHR treated with L732138. G. Band densities from NK-1 receptor detection in F were normalized to GAPDH in WKY (black bar, n=5), SHR (white bar, n=5), and SHR treated with L732138 (grey bar, n=5).