Fig. 4. Abnormal proliferation of GCPs in conditional Kif3a and Ftm mutant mice.

BrdU staining of a control (A, D, G), a conditional Kif3a mutant (B, E) and a Ftm mutant (H) cerebellum at E16.5 (A-B) and E18.5 (D-E, G-H). Rostral is to the right. Squares in D, E are shown at higher magnifications below. (C, F, I) Quantification of BrdU⁺ cells per 1000 μm² in regions I to IV and in the RL. Note that at E18.5, the number of BrdU⁺ cells is much smaller in the rostral part of the cerebellum in conditional Kif3a and Ftm mutants compared to wild type, while no significant difference was observed in caudal regions of conditional Kif3a and Ftm mutants. The BrdU⁺ cells were counted from at least three sections from each mouse at comparable mediolateral levels. Data from three mice per group were pooled for statistical analysis with Student’s t-test. **: p<0.01. Scale bars: 100 μm (A, B), 200 μm (D-E, G-H).