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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: Dev Biol. 2008 Mar 4;317(1):246–259. doi: 10.1016/j.ydbio.2008.02.026

Fig. 6. Abnormal Shh-induced proliferation of GCPs in cerebellar cultures from conditional Kif3a mutants.

Fig. 6

BrdU staining on cerebella sagittal slices from P3 wild type (A, C) and mutant mice (B, D) cultured with no stimulus (A-B) or 3 μg/ml Shh-N (C-D). BrdU staining (green) was counterstained with DAPI (blue) and visualized using confocal microscopy. (E) Quantification of the percentage of BrdU+ and DAPI+ nuclei in wild type and conditional Kif3a mutant slices with and without Shh-N. (F-G) Proliferation of isolated GCPs in aggregates obtained from P1 Kif3a mutants (G) compared to controls (F) with Shh-N for 48h. (H) Quantification of aggregate radius in wild type and conditional Kif3a mutants after 48h. Data from three mice per group were pooled for statistical analysis with Student’s t-test. ***: p<0.001. Scale bar: 100 μm.