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. 2014 Jun 3;9(6):e98178. doi: 10.1371/journal.pone.0098178

Figure 5. Soluble expression level of screened VH domains.

Figure 5

SDS-PAGE of soluble (A) and insoluble (B) fractions of E. coli expressing each VH domains selected from the frame-mutation library of MG2x1 were loaded as follows: 1, VHH (camel single-domain antibody); 2, HEL4; 3, MG2x1; 4, MG8-14; 5, MG2-47; 6, MG2-55; 7, MG2-57; 8, MG2-59; 9, MG4-2; 10, MG4-5; 11, MG4-6; 12, MG4-7; 13, MG4-12; 14, MG4-13; 15, MG4-17; 16, MG4-20; 17, MG4-28; 18, MG4-32; 19, MG4-33; 20, MG8-4; 21, MG8-5; 22, MG8-6; 23, MG8-8; 24, MG8-11; 25, MG8-12; 26, MG8-13. Lanes labeled ‘MW’ contained protein size markers (10 and 15 kD). U indicates fractions from a culture with no isopropylβD-1-thiogalactopyranoside.