Skip to main content
. 2014 Jun 3;9(6):e98178. doi: 10.1371/journal.pone.0098178

Figure 10. Validation of the combinatorial CDRH synthetic library built on MG8-14 scaffold.

Figure 10

SDS-PAGE of soluble and insoluble fractions of E. coli expressing VH domains selected randomly from the combinatorial CDRH3 synthetic libraries. Coomassie-stained gels are aligned by lane numbers (columns) and amino acid lengths of CDRH3 (rows). Images depict the region of the gel corresponding to the size of VH. Some images were combined with separate gels for the purpose of alignment (indicating with a dividing bar between gels). ‘MW’ indicates the protein size marker corresponding to a molecular weight of 15 kD.