Table 2. Frequency of mutations of the randomly chosen VH domains after screening of the combinatorial frame-mutation library of MG2x1 via TAPE.
| Mutations | Q39 | L45 | W47 | A50 | Y58 | A60 | Codon Degeneracy (%)a |
| F | 4 | 5 | 3 | 3 | 3.13 | ||
| W | 2 | 1 | 5 | 16 | 24 | 3.13 | |
| Y | 3 | 5 | 1 | 2 | 3 | 3.13 | |
| A | 2 | 2 | 3 | 1 | 7 | 6.25 | |
| C | 2 | 1 | 2 | 5 | 7 | 3.13 | |
| I | 4 | 4 | 4 | 3.13 | |||
| L | 3 | 7 | 3 | 6 | 3 | 1 | 9.38 |
| M | 3 | 1 | 6 | 2 | 1 | 3.13 | |
| P | 6 | 4 | 6.25 | ||||
| V | 5 | 5 | 6 | 3 | 1 | 1 | 6.25 |
| G | 1 | 1 | 6.25 | ||||
| N | 3 | 1 | 4 | 3.13 | |||
| Q | 3 | 3.13 | |||||
| S | 1 | 1 | 1 | 1 | 6 | 9.38 | |
| T | 3 | 4 | 2 | 1 | 3 | 6.25 | |
| H | 2 | 2 | 3.13 | ||||
| K | 2 | 1 | 3.13 | ||||
| R | 1 | 2 | 1 | 2 | 4 | 9.38 | |
| D | 4 | 3.13 | |||||
| E | 1 | 3.13 | |||||
| Total | 41 | 41 | 41 | 41 | 41 | 41 |
a
Possible substitution of amino acids into the frame-mutation library (%) according to the given codon degeneracy of primers used for mutagenesis of MG2x1 as shown in the Table S1.