Table I.
Gene | Sequences | Annealing temperature (°C) | Fragment size (bp) |
---|---|---|---|
TLR3 | Sense: 5′-GTCCAACGGCTTTGACGAGAT-3′ Antisense: 5′-CTGGCCCGAAAACCTTCTTCT-3′ |
56 | 188 |
TLR4 | Sense: 5′-TGTCCTCCCACTCCAGGTAAGT-3′ Antisense: 5′-GATTGCTCAGACCTGGCAGTT-3′ |
55 | 144 |
GAPDH | Sense: 5′-TCATGGGTGTGAACCATGAGAA-3′ Antisense: 5′-GGCATGGACTGTGGTCATGAG-3′ |
57 | 111 |
TLR, Toll-like receptor.