Figure 4.

Effects of BIIB021 on the cell cycle in SKM-1 cells are associated with the inhibition of cellular proteins, including CDK4/6 and cyclin D1. (A) Following the BIIB021 treatment, the SKM-1 cells were stained with PI and fixed with ethanol. The cell cycle phase was determined by flow cytometric analysis. The data represent one of three independent experiments with the same results. (B) The cells were treated with BIIB021 at the indicated concentrations for 24 h, whole cell extracts were prepared and western blot analysis was performed using specific antibodies. CDK, cyclin-dependent kinase; PI, propidium iodide.